Effects of ethylene glycol monomethyl ether [EGME] on male albino rat liver, kidney and femoral bone

ABSTRACT

Twenty male albino rats [100-120] gm body weight were used in this study and divided into a control and an experimental group, ten rats for each. Each rat from the experimental group was given, orally 80 mg / kg. body weight EGME every other day for 21 days. 5 mm. thick paraffin sections from the liver, kidney and decalcified femoral bone for each rat were prepared and stained by Hx and E, alcian blue PAS and Mallory triple stain. The diameter of epiphyseal cartilage was also measured both of control and experimental group. EGME induced marked histopathological alteration in the liver and kidney. Hepatic lesions were characterized by mild increase of mononuclear cell infiltration along the hepatic cords, dilation of some hepatic sinusoids and swollen hepatocytes. Renal lesion was in the form of glomerular hypercellularity and lobulation, also the renal tubules were dilated and had irregular luminal border. The femoral bone revealed atrophy of epiphyseal cartilage with disturbing cartilage columns and widening of the spaces of the matrix between them. Alcianophilic staining ability of the chonchocytes and matrix markedly declined. The trabecular bone also was markedly reduced in size. It was concluded that one should be cautious while using EGME containing materials such as some paints, cleaners, dyes, inks, resins and varnishes