Simultaneous determination of hydrochlorothiazide in combination with amiloride HCI or with spironolactone

ABSTRACT

Spectrophotometric and spectro-densitometric methods were used for the analysis of mixtures of hydrochlorothiazide with amiloride HCl or with spironolatone. In the spectrophotometric methods, the zero crossing of the second derivative spectrum was used for the determination of hydrochlorothiazide and amiloride HCl mixture at 252 nm and 387 nm, respectively, while, derivative ratio method was used for determination of hydrochlorothiazide and spironolactone mixture at [269 nm and 280 nm] and [237 nm and 246 nm], respectively. The proposed densitometric and spectrophotometric methods were applied to the analysis of laboratory prepared mixtures and pharmaceutical dosages forms containing hydrochlorothiazide with amiloride HCl or with spironolactone, and satisfactory results were obtained