Disposition kinetics of amoxicilliln from urinary excretion data in normal human volunteers in Pakistan

ABSTRACT

To investigate the disposition kinetics of amoxicillin trihydrate by urinary excretion data following oral administration in normal human volunteers. Design Addressed Evaluation of disposition kinetics of amoxicillin in local environment using urine data. Urine samples are easy to collect as compared with the blood samples and, therefore, urine data is helpful for the evaluation of disposition kinetics of drugs. Setting: Faculty of Veterinary Sciences, Agriculture University, Faisalabad-Pakistan and Department of Pharmacy, Islamia University, Bahawalpur-Pakistan. Subjects/The study was undertaken in seven normal human volunteers. Urine samples were collected over 12 hours following drug administration and analyzed for the drug by the disc diffusion method using Sarcina lutea as a test organism. The data was analyzed for computation of pharmacokinetic parameters by standard methods. A maximum amount of 29.2 +/- 3.0 mg of amoxicillin was excreted at four hours and 17.0 +/- 1.3 mg at 12 hours. The total amoxicillin cumulative amount excreted and% cumulative at the 12th hour was concluded to be 183.4 +/- 2.6 and 73.3 +/- 1.0%, respectively. Elimination rate constant of 0.60 +/- 0.03 hr and 1.18 +/- 0.05 hours in normal volunteers was observed in this study. Conclusions: Similarities and differences were both observed when the present findings were compared with the cited results. The urine data may be used as an alternative to the blood data for the estimation of pharmacokinetics of drugs