Effect of Losartan in ovalbumin sensitised guinea pig's bronchial tissue

ABSTRACT

To observe and evaluate response to antigen in presence of Losartan, in ovalbumin sensitized guinea pig's bronchial tissue. Design: Twenty five guinea pigs were sensitized with ovalbumin. Isolated strips of bronchial tissue from sensitized animals were used to observe and evaluate the response of ovalbumin in presence of Losartan. Settings Department of Pharmacology and Therapeutics, Basic Medical Sciences Institute, Jinnah Post Graduate Medical Centre, Karachi. Subject/Five strips of bronchial tissue from sensitized animal were used to determine EC., of ovalbumin. Each bronchial strip was individually incubated for 10 minutes in serial dilution of Losartan 10-5-10-Iglml and then exposed to ovalbumin EC50 Tissue response was recorded on Grass polygraph machine. Losartan caused an overall decrease in antigen responsefrom control ovalbumin EC50 value in sensitized bronchial tissue, along with progressive increase in antigen induced response with increasing concentration of Losartan. Our findings suggest that as Losartan shows a less severe increase in bronchial hyper-responsiveness, it can be used selectively in low doses with caution in patients with history of asthma, until more clinical experience with Losartan is accumulated