Effect of feeding different diets on the crude protein content and free amino acids in the haemolymph of Lucilia sericata [Meigen] and chrysomya albiceps [wied] prepupae [diptera-calliphoridae]

ABSTRACT

Larvae of both blowflies Lucilia sericata and Chrysomya albiceps were fed bovine meat, bovine liver, fish and chicken. The content of crude protein in the haemolymph of L. sericata prepupae when its larvae were fed on the four different diets, showed higher concentration when fed on bovine liver, than when fed on chicken and bovine meat. The least protein content recorded was when larvae were offered fish as diet. Meanwhile the protein content in the haemolymph of C. albiceps prepupae showed slightly higher concentration when larvae were fed on bovine liver than when fed on bovine meat, chicken and fish. Using [HPLC], the concentration of histidine was the most predominant amino acid in haemolymph of L. sericata prepupae when its larvae were fed on bovine liver followed by threonine when its larvae were fed on fish. While the amino acid leucine and serine were significantly low when larvae were fed bovine liver. The amino acid threonine in haemolymph of C. albiceps prepupae was high when its larvae were fed bovine meat and chicken and cysteine was significantly high in case of larvae fed on bovine liver and fish. The concentration of the amino acid glutamic acid was the least when fish was offered as diet and the amino acid isoleucine and leucine were significantly low when larvae were fed bovine liver