Immunohistochemical study of cell proliferation and tumor suppressor gene status in hyperplastic and neoplastic prostatic lesions

ABSTRACT

Predicting the clinical behavior of prostatic carcinoma could be difficult. One approach is to identify the molecular prognostic markers, therefore we evaluate the proliferative rate [MIB-1 antibody] and expression of tumor suppressor gene [P[53] protein] in 30 cases of prostatic adenocarcinoma. Values were correlated with histo-pathological parameters [Gleason score, stage and age of the patients]. Results were compared with the same studies on benign prostatic hyperplasia [n = 2], adenosis [n = 5], prostatic intraepithelial neoplasia [n = 5]. MIB-1 proliferation index was lowest in BPH, followed by adenosis,low grade [PIN], low grade cancer, high grade PIN and high grade cancer. Results of our study showed that MIB-1 proliferation index is highly correlated with tumor grade and clinical stage with percentage 100% and 62.5% respectively. As regard the expression of P[53], our results proved that it was confined to prostatic cancer especially high grades with overall incidence 70%. Also, it was found, that P[53] expression was correlated with tumor grade with incidence [83.3%], clinical stage [87.5%] and patients age> 50ys [75%]. These results provide additional evidence that adenosis is a histological small acinar proliferation more akin to BPH than high-grade PIN or adenocarcinoma.While high grade PIN showed strong correlation with prostatic carcinoma according to cell proliferation. Abbreviations [PIN] prostatic intraepithelial neoplasm, [BPH], benign prostatic hyperplasia