Genotoxic effect of diethylaminoethanol in mouse bone-marrow and germ cells

ABSTRACT

The genotoxic potential of the aliphatic amine diethylaminoethanol [DEAE] wasevaluated in vivo using two cytogenetic parameters, chromosomal aberrations inboth somatic and germ cells and sister chromatid exchanges [SCEs]. Theprotective effect of Royal Jelly was also tested. Male Swiss mice weretreated by gavage with DEAE at the dose levels of 100, 150 and 200 mg/kg b.wt. The used doses induced a significant percentage of chromosomalaberrations at 24 hours post-treatment, even after excluding the number ofmetaphases with gaps compared with control. Repeated treatment for three days withthe same dose induced a statistically higher percentage of chromosomalaberrations than single treatment. DEAE induced also a statisticallysignificant percentage of chromosomal aberrations in mouse spermatocytes atdiakinesis metaphase I after single and repeated treatments. The maximumpercentage of the induced aberrations reached 17.06 + 0.95 after repeatedtreatment with the highest tested dose for 3 consecutive days compared with4.8 +/- 0.33 for the controls. DEAE was found to potent inducer of SCEs atthe tested doses 100, 150 and 200 mg/kg b. wt. [P <0.01]. The meanfrequency reached 10.53 +/- 0.38, 12.97 +/- 0.41 and 15.79 +/- 0.52,respectively, compared with 5.54 +/- 0.48 for the controls. Treatment withRoyal Jelly at 1 mg/kg b. wt., decreased the percentage of totalchromosomal aberrations and the frequency of SCEs induced by DEAE in bonemarrow and spermatocyte cells