Application of riboflavin-deficient rat erythrocytes in the in vitro assay of faba bean toxicity
IJMS-Iranian Journal of Medical Sciences. 2002; 27 (2): 80-84
in English
| IMEMR
| ID: emr-59469
ABSTRACT
Faba bean toxicity [favism] is a common condition observed in our region. To develop a short and simple technique involving the use of riboflavin-deficient rats to test in vivo faba bean toxicity. Methods/Sprague Dawley rats were maintained on a riboflavin- deficient diet and their vitamin B2 status was monitored by the assay of erythrocyte glutathione reductase [EGR]. Their blood was found to be sensitive enough as early as 5 weeks of eating the deficient diet. Initially RBC preparations from the riboflavin-deficient rats were used to test the toxicity of different concentrations of divicine [0.0-13.5 microM]. It was found that an increase in divicine concentration resulted in an appreciable drop in reduced glutathione [GSH] of RBC. The assay procedure was then used to test the effectiveness of certain detoxification treatments of faba beans. A high correlation [r= 0.9] was noted between residual vicine [the main favism factor] concentration and the toxicity index [TI] of treated samples indicating the suitability of the above procedure for toxicity assay. It is recommended to use blood from Sprague Dawley rats that have been fed a riboflavin-deficient diet for about 5 weeks or longer, in the bioassay of toxicity of faba beans and their isolated favism principles
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Riboflavin Deficiency
/
Rats, Sprague-Dawley
/
Erythrocytes
/
Fabaceae
Limits:
Animals
Language:
English
Journal:
Iran. J. Med. Sci.
Year:
2002
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