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Comparison of PCR amplification of the FIM a gene and cultural method for detction of salmonellae in food and water
Veterinary Medical Journal. 2002; 50 (4): 627-638
in En | IMEMR | ID: emr-61154
Responsible library: EMRO
Bacteriological examination of local and imported feedstuff samples revealed that 11 out of 600 feedstuff samples harboured salmonellae with un incidence 1.8%. The highest incidence of isolation was in imported bone and meat meal [4%]. A polymerase chain reaction was performed by amplification fim A gene as specific method for detection of salmonella in feed and water. In order to obtain higher specificity, we have selected a series of primers internal to the fim A gene sequence. The concordance percent between conventional cultural method and PCR of feed and water were 96.6% and 100% respectively. This assay enable to detect 10 C.F.U. / 100 ml water and 10 C.F.U. / 25 g feedstuffs and it could detect only Salmonella strains. The detection of all position samples and the failure to amplify the fragment from non Salmonella strains confirm that the fim A gene contain sequences unique to Salmonella genus and demonstrate that this gene is suitable PCR target for detection of Salmonella bacteria. This rapid assay provides a sensitive and specific means of screening drinking water samples as well as feedstuff samples for the presence of Salmonella spp
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Index: IMEMR Main subject: Salmonella Food Poisoning / Salmonella typhimurium / Water Microbiology / Water / Polymerase Chain Reaction / Culture Media / Food / Food Microbiology Language: En Journal: Vet. Med. J. Year: 2002
Search on Google
Index: IMEMR Main subject: Salmonella Food Poisoning / Salmonella typhimurium / Water Microbiology / Water / Polymerase Chain Reaction / Culture Media / Food / Food Microbiology Language: En Journal: Vet. Med. J. Year: 2002