Comparison of PCR amplification of the FIM a gene and cultural method for detction of salmonellae in food and water
Veterinary Medical Journal. 2002; 50 (4): 627-638
in En
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| ID: emr-61154
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Bacteriological examination of local and imported feedstuff samples revealed that 11 out of 600 feedstuff samples harboured salmonellae with un incidence 1.8%. The highest incidence of isolation was in imported bone and meat meal [4%]. A polymerase chain reaction was performed by amplification fim A gene as specific method for detection of salmonella in feed and water. In order to obtain higher specificity, we have selected a series of primers internal to the fim A gene sequence. The concordance percent between conventional cultural method and PCR of feed and water were 96.6% and 100% respectively. This assay enable to detect 10 C.F.U. / 100 ml water and 10 C.F.U. / 25 g feedstuffs and it could detect only Salmonella strains. The detection of all position samples and the failure to amplify the fragment from non Salmonella strains confirm that the fim A gene contain sequences unique to Salmonella genus and demonstrate that this gene is suitable PCR target for detection of Salmonella bacteria. This rapid assay provides a sensitive and specific means of screening drinking water samples as well as feedstuff samples for the presence of Salmonella spp
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Index:
IMEMR
Main subject:
Salmonella Food Poisoning
/
Salmonella typhimurium
/
Water Microbiology
/
Water
/
Polymerase Chain Reaction
/
Culture Media
/
Food
/
Food Microbiology
Language:
En
Journal:
Vet. Med. J.
Year:
2002