Determination of aflatoxin m1 in powdered milk consumed by infants in Jeddah, Saudi Arabia using three methods

ABSTRACT

Thirty one samples of infant powdered milk formula marketed in Jeddah, Saudi Arabia were collected from different pharmacies and supermarkets then screened for aflatoxin M1 using three different techniques including thin layer chromatography, high performance liquid chromatography, and radioimmunoassay. These methods showed various results. Aflatoxin M1 was not detected in the samples by radioimmunoassay procedure while both thin layer chromatography and high performance liquid chromatography showed positive results. Aflatoxin M1 was detected in nine samples. Twenty two% of the positive samples recorded high levels of aflatoxin M1. The levels detected by the high performance liquid chromatography ranged from 67.7 to 173.0 ng/kg