Simultaneous determination of some anticonvulsants in some pharmaceutical preparations and in serum by high-performance liquid chromatography

ABSTRACT

The separation and determination of some anticonvnlsant drugs were done in some pharmaceutical preparations and in serum by high-performance liquid chromatography reverse phase. The chromatographic condition were mobile phase Acetonitril - Methanol - 0.06 M Potassium phosphate 25 15 60 W/W pH 4.0, column of octadecyle 5 mico [250 mm x 46 mm] Nucleosil with guard column. The extraction method was based on pharmacopia procedures for pharmaceutical preparations, and the technique used solid / liquid [SEP] for serum. This method permits the Simultaneous determination of all these compounds in pharmaceutical forms for different companies and in serum. Our method is simple and sensitive, based on qualitative and quantitative studies, the Coefficient regression was 0.9998 and Coefficient of variation was between 0.46% - l.t7% and the limit of detection was between 0.9%. Consequently our results showed a very good correlation and they were useful for quality control in the drug industries, the poisoning control centers and in medical laboratories