study on the effects of prenatal administration of valproic and [Depakine] on the lumbosacral region of the newly born mice

ABSTRACT

The present study aimed to demonstrate the embryotoxicity of valproic acid [depakine] [VPA] on the lumbosacral region and to determine the period[s] of susceptibility to produce its hazards. A total number of 40 newly born mice was used. They were divided into a control and three experimental groups. The experimental mothers were injected with 400 mg/kg body weight of sodium valproate three times daily at the 8th, 9th and 10th day of gestation, one day each group. Both control and experimental groups were examined for any external abnormality. The specimens were decalcified, cut at 10 microns thickness and stained by hematoxylin and eosin. Staining of the newly born mice was processed for alizarin red stain. From the results obtained it was concluded that, when VPA must be used during pregnancy, it should be used with strict medical supervision and after closure of the posterior neuropore