Cytogenetic evaluation of in-vitro matured buffalo oocytes in different culture conditions

Ocytes were collected by aspiration from ovaries of slaughtered buffaloes. A total of 1240 oocytes were selected for in vitro maturation [IVM] procedure in culture media for 22- 24 hr and 25 -28 hr at 39°C in 5% CO[2] incubator. For both cultured periods oocytes were matured in droplets of TCM-199 + estrous buffalo serum [EBS], TCM-199 + foetal calf serum [FCS], Ham's F-10 + EBS and Ham's F-10 + FCS. Cytogenetic analysis of 582 oocytes cultured in the four media conditions in both periods of time showed wide range of meiotic configurations : metaphase-I, anaphase-I, telophase-I and metaphase-11. Also, the cytogenetic analysis identified matured oocytes at metaphase-II with diploid number of chromosomes [3.95%]. Analysis of variance showed that, the maturation rate in the two media and sera didn't differ significantly. The maturation rate to the second metaphase were significantly higher [P0.01] at 25 - 28 hr than in the same media for 22 - 24 hr period of time