Cystatin capture-dot-enzyme-linked immunosorbent assay for immunodiagnosis and assessment of cure of experimental trichinellosis in mice
Journal of the Egyptian Society of Parasitology. 2003; 33 (1): 275-90
in English
| IMEMR
| ID: emr-62841
ABSTRACT
Cystatin capture-dot-enzyme-linked immunosorbent assay [CC-dot ELISA] was evaluated as a new version of ELISA for the diagnosis of trichinellosis by the detection of anti-Trichinella spiralis cysteine proteinase [CyP] IgG using nitrocellulose membrane sensitized with cystatin as a capture reagent for CyP from T. Spiralis muscle larvae excretory secretary products [ESP] without purification compared with the detection of anti-T. spiralis IgG by conventional [conv.] ELISA using whole ESP. Experimentally infected mice with light [100 larvae/mouse] and heavy [300 larvae/mouse] infections by T. spirals larvae at 7, 14, 21 and 56 days post-infection and after flubendazole treatment were examined
Search on Google
Index:
IMEMR (Eastern Mediterranean)
Main subject:
Trichinellosis
/
Immunologic Tests
/
Enzyme-Linked Immunosorbent Assay
/
Cysteine Endopeptidases
/
Sensitivity and Specificity
/
Larva
/
Mice
/
Antibodies
Limits:
Animals
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2003
Similar
MEDLINE
...
LILACS
LIS