Effect of carnosine administration on the immune response of rabbit to schistosoma mansoni antigens

ABSTRACT

In this study, sodium dodecylsulfate polyacrylamide gel electrophoresis [SDS-PAGE] separation of soluble worm antigen preparation [SWAP], cercarial antigen preparation [CAP] and soluble egg antigen [SEA] of Schistosoma mansoni showed obvious qualitative and quantitative differences. The shared polypeptides of the three stages of S. mansoni were 116, 72.768 and 32.367 kDa under reducing conditions. The different anti-sera raised in rabbits against the different stages of antigens were recognized by electro-immune transfer blotting [EITB]. Each of the three groups separated eight bands. Carnosine treatment of rabbits immunized with SWAP, CAP or SEA resulted in the disappearance of two bands in SWAP group and one band in CAP group in comparison with the non-treated immunized groups. This indicated that the carnosine modulated the immune response of rabbits against S. Mansoni antigens