Antisense RNA to the type I insulin-like growth factor receptor reversed the transformed phenotype of PC-3 human prostate cancer cell line in vitro
Medical Journal of the Islamic Republic of Iran. 2003; 17 (1): 59-66
in English
| IMEMR
| ID: emr-63503
ABSTRACT
The insulin-like growth factor I receptor [IGF-IR] plays an essential role in the establishment and maintenance of transformed phenotype. Interference with the IGF- IR pathway by antisense causes reversal of the transformed phenotype in many rodent and human tumor cell lines. We stably transfected the PC-3 human prostate cancer cell line with an IGF-IR antisense RNA expression plasmid. The number of lGF-I receptors on the antisense-transfected PC-3 cells was reduced by 40.2% relative to the control-transfected cells. The transfected cells maintained their high expression of IGF-IR antisense RNA for up to one year in selective medium. The reduction in the expression of IGF-IR had no effect on the cell growth in monolayer. The clonogenicity of antisense-transfected cells was 24.7% of the clonogenicity of control-transfected cells in soft agar. There was a good correlation between IGF-IR level and inhibition of transformation in soft agar. These results indicate that reduction of IGF-IR by antisense RNA can reverse the transformed phenotype of human prostate cancer cells
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Phenotype
/
Cell Line, Transformed
/
Tumor Cells, Cultured
/
RNA, Antisense
Limits:
Humans
Language:
English
Journal:
Med. J. Islamic Rep. Iran
Year:
2003
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