Molecular diagnosis of helicobacter pylori in patients with chronic liver disease.

ABSTRACT

Fifty individuals were included in this study; 30 patients [group I] were diagnosed as liver cirrhosis and were subjected to injection sclerotherapy with follow up and 20 individuals with matched age and sex serving as normal controls [group II]. Beside the routine laboratory investigations, HP IgG, PCR for HP ure A and ure C were determined in the mucosal biopsy of both groups and PCR for HP ure A in the liver biopsy obtained from 18 patients of group I whose prothrombin time allowed the performance of liver biopsy. HP IgG was detected in 29 out of 30 liver cirrhosis patients and the highest incidence of HP IgG was found in patients over the age of 30 years and in those with gastric and duodenal erosions rather than peptic ulcer, while HP IgG was detected in 17 out of 20 cases of the control group. HP urec C was detected in 13 out of 30 gastric mucosal biopsy samples and HP urec A was detected in 23 out of 30 gastric mucosal biopsy samples. Concomitant HP urec A and C were detected in 9 out of 30 gastric mucosal biopsy samples. HP urec A was detected in all liver biopsy samples