protective effects of early treatment with propofol on endotoxin-induced acute lung injury in rats

ABSTRACT

To investigate the effects of treatment with propofol administration at different time point in acute lung injury of endotoxin-induced shock in rats. 76 male wistar rats were randomly assigned to five groups A] control group; B] endotoxemic group, receiving intravenous lipopolysaccharide [LPS] 8mgkg -1; C] pretreatment group, treated identically to endotoxemic group with the additional administration of propofol [5mgkg -1 bolus, followed by infusion at 10 mgkg -1h -1] of lhr prior to the injection of LPS; D] simultaneously treatment group, treated identically to endotoxemic group with the additional administration of propofol simultaneously with the injection of LPS; E] post-treatment group, which was treated identically to endotoxemic group except for administration of propofol lhr after the injection of LPS. PaO 2, pH, MAP and survival rate were recorded and plasma NO, TNF-alpha were measured during 5-hr after the injection of LPS. After the rats were killed, lung tissue was sampled to measured expression of inducible nitric oxide synthase [iNOS], nitrotyrosine [NT], myeloperoxidase [MPO] activity, malondialdehyde [MDA], wet-to-dry lung weight ratio [W/D], and pulmonary permeability index [PPI]. Compared with the endotoxemic group, both the pretreatment and simultaneously treatment groups, significantly improved PaO 2, pH, MAP and 5th hour survival rate of rats, and attenuated endotoxin-induced increased iNOSmRNA, NT expression, MPO activity and MDA level in lung tissue, and decreased pulmonary microvascular permeability, TNF alpha, NO in plasma. But these beneficial efficacies were blunted in the post-treatment group. These findings showed that propofol administration may provide protective effects on acute lung injury in endotoxin-induced shock