Drugs affecting wound healing and alkaline phosphatase activity

ABSTRACT

Alkaline phosphatase [AP] plays an important role in absorption, transport processes, and the availability of inorganic phosphates in normal tissues. In this study we found that human wound tissue contains AP activity which displayed typical enzymic properties. The enzyme had a Km of 9.3 x 10-5M and Vmax of1.098 moles/ml/min. The effect of various antimicrobial and anti-inflammatory drugs o the activity of AP in burn wound tissue was also examined. Low concentrations of oxytetracycline [OT, doxycycline hyalite [DH] and doxycycline polyphosphate sodium complex [DMSC] in increasing order of potency, inhibited AP activity in a dose-related manner. Other drugs, ampicillin, bacitracin, Zn, erythromycin, hydrocortisone, neomycine, polymeric B, sulphamethoxazole and trimethoprim did not inhibit AP activity at concentrations up to 5mM. in addition, isoenzymes of AP from different rabbits and human burn wound tissues were inhibited to varying extents by DH. On the other hand, DMSC selectively inhibited the human burn wound tissue AP activity and had no effect on AP derived from other tissues