anti-metastatic potency of gnidilatimonoein, from daphne mucronata, compared to two glycosylation inhibitors: castanospermine and tunicamycin

ABSTRACT

It is well known that the modification of oligosaccharide moieties of the cell surface glycoproteins modulates the adhesion and metastatic potential of several cancerous cell lines. Based on this knowledge, the anti-metastatic property of Daphne mucronata crude extract and one of its newly characterized active component, Gnidilatimonoein, were evaluated in wehi-164 cells by measuring their adhesion to fibronectin coated plates, relative to Castanospermine and Tunicamycin treated cells.Twenty four hours after treatment of the cells with the plant crude extract [equivalent to 0.54 mg of the plant leaves powder/ml], Gnidilatimonoein [0.94 nM], Castanospermine [2.6 micro M] and/or Tunicamycin [2.4 micro M], their attachment to fibronectin-coated wells were depressed, by 24%, 30%, 26% and 58%, respectively. This data may classify the new anticancer compound, Gnidilatimonoein, as a strong glycosylation inhibitor