Phytochemical and microbiology studies on Berberis tinctoria lesch

ABSTRACT

The stem bark, stem wood, root bark and root wood of Berbetis tinctoria Lesch. were extracted successively with various organic solvents and the subsequent fluorescence analysis was carried out. The quantitative ash values and extractive values were determined. Berberine was isolated and characterized. The quantitative estimation of berberine in the various extracts was carried out by HPTLC technique. The antibacterial and antifungal activities of methanolic extracts were carried out by cylinder-plate and serial dilution method. The methanolic extracts showed prominent activity against Staphylococcus aureus in comparison to control [Ampicillin trihydrate]