effect of gnidilatimonoein from daphne mucronata, on the adhesive property of human platelets

ABSTRACT

The adhesive interaction between tumor cells and the host cells or the extracellular matrix plays a crucial role in tumor metastasis. To evaluate the mediation of cell adhesion by Daphne mucronata, an anti-cancer medicinal plant in Iranian folk medicine, the adhesion of thrombin activated human platelets to the cultured monocytes or HL-60 cells was investigated under the effect of the plant extract [0.54 mg/ml] or one of its purified components [gnidilatimonoein, 0.94 micro M]. Treatment of the platelets with the plant extract or the active component, for various time intervals, followed by their activation by thrombin resulted in 80-90% reduction in the number of monocytes with more than 10 attached platelets. Similarly, under almost all identical conditions, the adhesion of the activated platelets to HL-60 cells was decreased by 90%. The adhesion of thrombin activated human platelets to the plant treated HL-60 cells was also reduced significantly [by 95%]. These data clearly indicate that Daphne mucronata is capable of mediating tumor metastasis through effecting cell adhesion properties of the cells