[Affect of three antibacterial mouthwashes chlorhexidine 0.2%, povidine iodine 1% and cetyl pyridinum chloride 2.5% on bacterials strep cultures]

ABSTRACT

Dental plaque is the main cause of periodontal disease. Prevention and treatment of periodontal disease is provided by inhibition of plaque formation or its removal. Uses of Mouthwashes are one of the methods for plaque control. The purpose of this study was to determine the most efficient mouthwash in prevention of plaque formation. This experimental study consisted of 48 microbial culture. Antibacterial effect of three well known mouthwash povidine iodine 1%, chlorhexidine 0.2% and cetyl pyridinum chloride 2.5% were studied on two bacterial species strep mutans and bacterial melaninogenicus, which were obtained from dental plaque of three different patients. This mentioned bacteriac were added to culture mediums until the turbidity equivalent of number 0.5 macfarland tube was achieved Then mouthwashes were added to the cultures which were then placed in 37°C incubator in two different conditions, aerobic and anaerobic. The turbidity of the aerobic and anaerobic cultures were compared with standard Macfarland tubes after 24 and 72 hours respectively. Data analysis was done with ANOVA, kroscal valis and T-test. The mean number of streptococus mutans in one milliliter of the culture medium, with mouthwashes including povidine iodine 1%, chlorhexidine 0.2% and cetylpyridinum chloride 0.5% added was determined. 19.5x10[8], 0.19x10[8] and 0. 75x10[8] respectively and for bacteroid melaninogenicus 12.75 x10[8], 0, 0.56 x10[8] was achieved respectively. Total average of bacterial number in one milliliter of culture, when mouthwashes were added was determined 16.1 x10[8], 0.09 x10[8] and 0.65 x10[8] respectively. Result of this study showed that for each type of species and also in general, antibacterial effect of three applied mouthwashes in descending order was chlorhexidine 0.2%, celypyridmum chloride 2.5% and povidine iodine 1%. In other word chlorhexidine can be suggested as elective mouthwash. Finally longitudinal study, invivo-invitro, with application of more mouthwashes and bacterial species is suggested