Effect of largely wide dosage ranges of acetyl salicylic acid, diclofenac and piroxicam on interleukin-3 [IL-3] production

ABSTRACT

Interleukin-3 [IL-3] is a multi-lineage cytokine affecting myeloid and lymphoid lineage as well as megakeryocytes. It is regarded as beneficial cytokine in various stages of pregnancy. It plays a key role in contact sensitivity and in delayed type hypersensitivity reaction. The effect of acetyl salicylic acid [ASA] on IL-3 production is a subject of controversy. Some studies demonstrated an ASA inhibiting action on IL-3 production, while others point to an enhancement effect. The present study aims at delineating the possible different effects of ASA on IL-3 production through testing wide range of doses, namely 1 to 500 mg/kg., p. o., in adult male Swiss mice. In addition, the study included testing wide range of doses of two other nonsteroidal anti-inflammatory drugs [NSAIDs], which are diclofenac [0.04 to 20 mg/kg., i. m.] and piroxicam [0.006 to 3 mg/kg., i. m.]. Parallely, changes in each of bone marrow lymphocytes count, total white blood cells count, and thymus and spleen relative weights were evaluated. Animals received each of the three NSAIDs daily for 7 days. Twenty four hours after last dosage, animals were proceeded for the above mentioned assessments. The obtained results revealed that all tested NSAIDs have the tendency to increase IL-3 production. They exhibited different dose-response patterns for elevation of serum IL-3 levels. Same phenomenon was evident regarding all other investigated parameters. The study confirms existence of largely dose-dependent variable effects of ASA and other NSAIDs on ILs-3 production as well as on the other evaluated parameters, in mice