Evaluation of capillaria philippinensis coproantigen in the diagnosis of infection

ABSTRACT

In order to overcome the false negative diagnosis of infection with C. philippinensis in the absence of eggs in stool, coproantigen prepared from the stools of infected patients was evaluated serologically. This antigen was able to detect anti-Capillaria antibodies in the sera of infected cases at the same OD level produced with Capillaria crude worm antigen using indirect ELISA technique. C. Philippinensis coproantigen did not cross-react with the sera from patients with Schistosomiasis mansoni, Fascioliasis or Strongyloidiasis at 100 serum dilution. Laboratory-prepared hyperimmune sera versus crude worm antigen of C. philippinensis succeeded in capturing Capillaria antigen prepared from the stools of infected patients and did not cross-react with the coproantigens prepared from the stool samples of cases infected with S. Mansoni or Fasciola using sandwich ELISA technique