Detection of genetic variabiltiy in non - human isolates of fasciola hepatica and Fasciola gigantica by the RAPD - PCR Technique
Journal of the Egyptian Society of Parasitology. 2004; 34 (2): 679-89
in English
| IMEMR
| ID: emr-66766
ABSTRACT
The present study showed the molecular characterization of Fasciola gigantica and F. hepatica isolates collected from cows and sheep, using the random amplified polymorphic DNA fragments-polymerase chain reaction [RAPDs-PCR] technique. Optimal standardization of amplification conditions and thermocyclation were made, using genetic markers. The methodology used compared the genetic patterns of the two species [interspecies] and inside each species [intra-species] between cow and sheep and the amplification fragments were between 135 and 741 base pairs of marker. The results showed genetic variations [polymorphisms] of Fasciola gigantica and F. hepatica with amplification fragment based on a 500-400 base pair [bp]. Inside each species, there were genetic variations in bovine and ovine and the amplification fragments were between 600 and 400 base pairs [bp]
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
DNA
/
Cattle
/
Sheep
/
Polymerase Chain Reaction
/
Fasciola
/
Fasciola hepatica
Limits:
Animals
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2004
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