Molecular identification of coproantigens of Cryptosporidium parvum compared to conventional staining techniques
Journal of the Egyptian Society of Parasitology. 2004; 34 (3): 967-78
in English
| IMEMR
| ID: emr-66788
ABSTRACT
To compare the immunologic techniques with the conventional staining methods [mainly modified Ziehl-Neelsen, MZN], 93 children [65 immunocompromised and 28 immunocompetent] potentially at risk of Cryptosporidium parvum were studied. Using MZN, a prevalence of 10.7% in diarrheic children was found. ELISA coproantigen and detection of 23 kDa band of immunoblotting by serum IgG were sensitive and specific. They gave 85.7% sensitivity, 100% specificity, 100% diagnostic accuracy, 100% positive predictive value and 98.9% negative predictive value. ELISA detection of serum IgG gave 85.7% sensitivity, 97.7% specificity, 96.8% diagnostic accuracy, 75% positive predictive value and 98.9% negative predictive value. So, it was concluded that 23 kDa band determined by ELISA is a valuable sensitive and specific mean of diagnosing cryptosporidiosis, as this antigen is a consistent target of the humoral immune response
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Child
/
Prevalence
/
Sensitivity and Specificity
/
Diarrhea
/
Microscopy
/
Antigens, Protozoan
Type of study:
Prevalence study
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2004
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