Enhancement of fracture healing in crossbred dogs using subcutaneous injection of salmon calcitonin

ABSTRACT

The impaired fracture healing results in pseudoarthrosis or skeletal deformity that causes functional disability bone is composed of cells and organic matrix [30%], and minerals [70%]. Fracture healing consists of three interrelated phases inflammatory, repair, and remodeling, to return to original tissue structure. To asses the effect of salmon calcitonin on fracture healing during the mineralization process. Twenty crossbred dogs were used in this study. After a fracture was created at the midshaft of the radius and ulna of the animals and stabilized with an external splint. Ten dogs of them were injected subcutaneously with salmon calcitonin. Blood samples were taken from the cephalic or recurrent tarsal vein before the operation and at 2,4, and 6 weeks after the operation. For histological assessment bone biopsy was taken from the fractured sites at 2,4,6 weeks post-operative from both groups. The radiographic analysis revealed complete union at 6 weeks post fracture fixation in the salmon calcitonin treated group [2]. In the other group the fracture remained unhealed till the 10[th] week post-operative. The serum biochemical analysis showed highly significant increase in alkaline phosphatase in group [2] and non significant decrease in calcium level in the group [1]. The histopathological examination. Declared that the periostium was activated, the osteoblasts appeared early and the formation of osteoid callus was noticed at the 4[th] week. At the 6[th] week complete ossification and formation of the boney specules were observed in the group [2]. While in the group [1] all these observations were delayed 4-6 weeks. Salmon calcitonin accelerates fracture healing and therefore proposes that it is a potent bone anabolic agent for clinical use. It also gives us simple and easier interferences by using of external fixation rather than using of the most massive, complex, and expensive internal fixations for treating the simple, single long bone fractures in dogs. Fracture healing, salmon calcitonin, dogs