Digital image analysis as an alternative method for photometric and spectrophotometric measurements

ABSTRACT

The author proposes a new and easy low cost system as an alternative method for the classical apparatus usually used in the field of photometric and spectrophotometric measurements. The proposed system depends on digital image analysis and it is composed from an imaging unit [Scanner or Camera CCD] computer, capillary tubes support, software [especially programmed for this purpose] and a printer. The author demonstrates the different functions of this system such as a spectrophotometer for colorimetric determination in visible light and he gives one of the phenols colorimetric determination as an example and as a densitometer in electrophoresis measurements for separated and stained serum proteins. A comparison of the same sample results with a classical densitometer [Helena] is exposed. In addition to the possibility of its application in T.L.C to determine quantitatively the separated spots, the use of this method in the field of fluorimetric measurements has been discussed