Protective role of diethyldithiocarbamate against toxicity of some new antibacterial and antitumor platinum[II] and [IV] organochelates

ABSTRACT

Aim: Five newly synthesized Schiff base complexes of the platinum [II] and [IV] were screened for their in vitro antibacterial and antitumor activity. Meanwhile, the protective role displayable by diethyldithiocarbamate [DDTC] against the inducible toxicity of two of platinum chelates was investigated as well. Their minimal inhibitory concentrations [MICs] were determined against narrow range of Gram negative and Gram positive bacteria while the antitumor potentials were tested against Ehrlich ascites carcinoma [EAC]. Half LD50 of the two selected antitumor complexes [naphthylaldehyde and diamino hexane [Pt [II] NDAH] and Pt [IV] NEDA] was i.p. injected in male Sprague Dawley rats then their biochemical consequences were investigated. The mode of action studies on intact cells of the most sensitive bacterium [Bacillus subtilis NRRL-B-4378] revealed that Schiff base Pt[IV] chelate derived from naphthylaldehyde and ethylene diamine [Pt [IV] NEDA] had reasonable reducing effects on microbial RNA and protein contents, while DNA was powerfully affected. Regarding their toxicity, Pt[IV] NEDA was found to be less hepato and nephro toxic than Pt[Il] NDAH. The hepatotoxic and nephrotoxic effects of these two platinum complexes seem to be reasonably ameliorated in animals post treated with DDTC as indicated by nearly restoration of normal values of the studied biochemical parameters