influence of lipid composition and beta-carotene on lipid peroxidation in liposomes

ABSTRACT

Oxidative damage to membrane lipid is one of the prime events occurring in aging and other undesirable physiological processes. In this study experiments were performed on liposomes [prepared either from crude erythrocyte phospholipids or purified egg yolk phosphatidylcholine] as models of lipid bilayer portion of biomembranes. The effects of beta-carotene, and phospholipid composition on peroxidation process, initiated by Fe[2+], were studied. It was found that beta-carotene does not show any noticeable antioxidant effect on the peroxidation process initiated by Fe[2+] in liposomes prepared from erythrocyte phosphatides, whereas it effectively suppressed the same process in egg yolk phosphatidylcholine [EYPC]. It is concluded that the anti-/pro-oxidant activity of beta-carotene is also dependent on the membrane lipid composition and this may provide an explanation about the conflicting reports on its role in ordinary or promoted oxidation experiments