Vitamin B6 production by some local fungi

ABSTRACT

The potential of twenty-eight fungal species belonging to 13 genera isolated and identified from Egyptian soils to release vitamin B6 in their culture filtrates were tested. It was found that nearly 60% of the tested fungi showed positive results as the production of vitamin B6. Aspergilli particularly Aspergillus tomarii Kita was distinguished by its capacity to produce vitamin B6 when grown on a selected medium. The maximum viamin B6 yields were achieved after on 8 days incubation at 30°C. An initial pH value of 6 was found to be the optimum for growth of A. tamarii and vitamin B6 production. The influence of aeration as well as the amount and age of inoculum upon biosynthesis of the vitamin B6 and biomass yield were also discussed briefly. The present paper gives an account of observation made on the production of vitamin B6 and biomass in relation to the carbon and nitrogen compounds supplied in the medium. Glucose followed by fructose then sucrose were more initiative for the growth and vitamin B6 production than cellulosic compounds. Sugar cane molasses represented the most favorable waste substrate than other sources invesigatored. Yeast extract followed by ammonium nitrate then casein were the best nitrogen sources for biomass and vitamin B6 production by tested fungus. In addition, the impact of the levels of selected carbon and nitrogen sources on both experimental parameters were also examined