Regulation of AD-D2 expression in schizosaccharomyces pombe

ABSTRACT

Great variability for xanthine accumulation was obtained among the strains of S. Pombe and among their derivatives by plating on LM medium supplemented with limiting amount, of adenine [10 mg/1] for one and 2 generations. They also showed great variability for induction and repression of the enzymes preceding the block. This variability was clearer at the lower concentrations of the end product. The diploids developed between different inducible and repressible strains in the adonine pathway showed that the majority of the regulator genes are dominant excluding the possibility of at least one recessive regulator gene and there are interactions among the adenine regulator genes. Tetrad and random spore analyses showed that the adenine reGulator genes are unlinked with probably similar effects on regulation of the adenine pathway enzymes. They also showed that there is no linkage between adenine regulator genes and other arg-1 or arg-10 which are unlinked