Column chromatographic fractionation of soybean phospholipid and chemical analysis of isolated phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidic acid and cerebroside

ABSTRACT

Phospholipid mixtures were isolated from crude soybean oil and laboratory prepared soy lecithin were fractionated on two silicic acid columns with subsequent thin layer chromatography identification of isolated fractions. The percentages of the isolated four eluted fractions from each of the two columns were calculated. Phosphatidylcholine, phosphatidylethanolamine phosphatidylinositol, phosphatidic acid and cerebroside were isolated. Fatty acid composition and total phosphorus of the isolated phospholipids were determined