HIF-1 Alpha and p53 as targets for crude unfractionated honey in affecting cancer cell death

ABSTRACT

C rude unfractionated honey was used to study its effects on two transcriptional factors HIF-l alpha and p53 at low and high concentrations using HEK293 [human embryonic kidney] and RCC4 [renal carcinoma] cell lines, while they kept under low protein abundant due to efficient 26s proteosomal degradation. The results of the present study showed that crude honey does not affect the stability of HIF- I alpha protein as seen by immunoblotting in normal HEK293,as well as p53 in comparison to actin which is applied as the loading control. On the other hand, the crude honey has been lowered the HIF- 1 alpha stability in RCC4 tumor cell line which was clearly seen with high concentration, while p53 showed no effect which may suggest impaired-cytoplasmic shuttling, the matter which may be a good sign for the ability of crude honey to initiate apoptotic pathways