Diagnostic role of ELISA detected antibodies to citrullinated antigens in rheumatoid arthritis patients: a comparative study
Egyptian Rheumatology and Rehabilitation. 2005; 32 (2): 135-146
in English
| IMEMR
| ID: emr-70561
ABSTRACT
To investigate rheumatoid arthritis [RA] for the diagnostic role of anti-cyclic citrullinated peptide antibody [anti-CCP] and antifilaggrin antibody [AFA] in comparison with RF and matrix metalloproteinase-3 [MMP-3]. A total of 60 RA patients and 50 other rheumatic disease patients were included in the study. We used a second generation enzyme-linked immunosorbent assay [ELISA] kit for the detection of anti-CCP. We constructed recombinant human filaggrin, which was citrullinated in vitro with human peptidylarginine deaminase, and subsequently used it as the coating antigen for AFA-ELISA. The specificities of anti-CCP [87.3%] and AFA [93.5%] were superior to those of RF [80.4%] and MMP-3 [50.1%]. The sensitivity of anti-CCP [86.3%] was superior to all others. However, the sensitivity of AFA [66.9%] was inferior to those of RF [68.5%] and MMP-3 [72.6%]. ELISA detection of antibodies to citrullinated antigens, especially a second generation anti-CCP, showed higher discriminative ability than other assays, including RF, and would be useful to aid in the diagnosis of RA in clinical practice
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
C-Reactive Protein
/
Enzyme-Linked Immunosorbent Assay
/
Comparative Study
/
Polymerase Chain Reaction
/
Citrulline
/
Matrix Metalloproteinase 3
/
Amylases
/
Antibodies
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Egypt. Rheumatol. Rehabil.
Year:
2005
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