Investigation of two recessive disorders in breeder bulls of Abbas Abad animal breeding center

ABSTRACT

To prevent distribution of recessive alleles in dairy herds all bulls used for AI [Artificial Insemination] have to be tested. In this study 26 blood and 4 semen samples were supplied from Iranian Holstein bulls used for AI. Genomic DNA was extracted from 100 ml of blood and 200 ml of semen. Samples were tested by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [PCR-RFLP] method. PCR reaction was performed for amplification of polymorphic region of the CD18 gene on chromosome 1 and exon 5 of ASS gene on chromosome 11. We detected one Bovine Leukocyte Adhesion Deficiency [BLAD] carrier and no carrier for bovine Citrolliemia in this study. Hardy-Weinberg test confirmed the equilibrium of BLAD locus in this population