Anticonvulsant activities of 7-phenyl-5H-thiazolo[5, 4-e][1, 2, 3, 4] tetrazolo[5, 1-c] pyrrolo[1, 2-alpha][1, 4] diazepine and 7-phenyl-5H-thiazolo[5, 4-e][1, 3, 4] triazolo[5, 1-c] pyrrolo[1, 2-alpha][1, 4] diazepines

ABSTRACT

Anticonvulsant activity of 7-phenyl-5H-thiazolo[5, 4-e][1, 2, 3, 4]tetrazolo[5, 1-c]pyrrolo[1, 2-a][1, 4]diazepine [5] and 7-phenyl-5H-thiazolo[5, 4-e][1, 3, 4]triazolo[5, 1-c]pyrrolo[1, 2-a][1, 4]diazepines [6a-d] was measured against pentylenetetrazole [PTZ]-induced seizures in mice. Intraperitoneal injections of different doses [12.5, 25 and 50 mg/kg, i.p.] of test compounds decreased PTZ-induced seizure significantly in a dose-dependent manner.The test compounds were administered 30 min befor PTZ [80 mg/kg, i.p.] injection. The maximum response was obtained with 50 mg/kg of compound 6d that showed more anticonvulsant activity compared to diazepam [0.5 mg/kg]. The frequency of mortality was also decreased by all listed compounds. Pretreatment of animals with flumazenile [as a benzodiazepine, BDZ receptor antagonist] decreased, but not completely inhibited the anticonvulsant activity of compound 6d [50 mg/kg]. These results indicate that besides BZD receptors, other neurotransmitter systems may be involved in anticonvulsant activity of the tested compounds