histologic evaluation of rat connective tissue reaction to three different gutta-percha cones [ariadent, roeko and diadent]

ABSTRACT

Regarding the manufacture of different gutta-percha cones and the importance of apical seal in a successful root canal therapy, this experimental study was designed to compare the invivo effects of Ariadent gutta-percha with Roeko and Diadent. In this exprimental invivo study, 29 mature Albinus rats [mean weight 202/77 gr] were selected. Four intradermal incisions were made in the dorsal part of each rat. In three incised areas, a piece of each gutta-percha was placed and one of the four incised areas was assumed as the negative control. Animals were sacrificed at 15, 30 and 60 day intervals and then specimens were taken from subcutaneous connective tissues of rats. All of the specimens were prepared histologically and stained using H and E staining method, histological assessment was based on the number of inflammatory cells. Data were analysed using One-Way ANOVA and Duncan test with 95% confidence level through SPSS software. The results revealed that Ariadent gutta-percha in 15 day interval was more reaction inducing than Roeko and Diadem but in 30 and 60 day intervals, tissue biocompatibility of Ariadent was the same as Roeko and Diadent. Iranian Ariadent gutta-percha can be considered as a substitute for other foreign gutta-percha cones