Serological diagnosis of trichinealla spiralis in experimentally infected mice

ABSTRACT

ELISA and IFA tests were used to detect IgG levels in sera of experimentally infected mice with Trichinella spiralis during a period of 12 weeks post infection. A crude saline extract of muscle larvae was used for application of ELISA technique, while the intact T. spiralis muscle larvae were used as an antigen for IFAT. In both tests, T spiralis IgG antibodies could be detected one week post infection in sera of all infected mice. ELISA test showed a sensitivity of 100% during the whole period of the experiment, while its specificity was 93.3%. Meanwhile, IFA test revealed a sensitivity of 100% and a specificity of 85%