Molecular characterization of Egyptian trichomonas vaginalis clinical isolates by HSP70 restriction fragment length polymorphism
Journal of the Egyptian Society of Parasitology. 2005; 35 (2): 699-710
in English
| IMEMR
| ID: emr-72361
ABSTRACT
Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism [RFLP] analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates [55%] showed the same RFLP pattern, teen of them [90.9%] were from symptomatic patients. T. vaginalis virus [TVV] was present in 7 isolates [35%]. Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymorphism, Restriction Fragment Length
/
DNA
/
Polymerase Chain Reaction
/
Genotype
/
Metronidazole
/
Molecular Biology
Limits:
Female
/
Humans
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2005
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