Characterization of specific trichomonas vaginalis target antigens from different isolates by immuno-blotting against hyperimmune rabbit serum
Journal of the Egyptian Society of Parasitology. 2005; 35 (3): 891-898
in English
| IMEMR
| ID: emr-72378
ABSTRACT
The SDS-PAGE and immunoblot methods were used to identify Trichomonas vaginalis specific target antigen[s]. Ten T. vaginalis isolates, cultured on TYM media, were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis [SDS-PAGE], revealed 34 distinct bands. Immunoblotting against a hyperimmune rabbit serum, showed 20 reactive bands ranging from 14-205 kDa. There was isolate to isolate variability among 8 isolates, while 2 isolates [6, 7] showed a similar antigenic patterns. Imunoblotting of the isolates showed a total of 20 molecular weight bands, 80% of isolates gave positive immunologic reactions above 100 kDa, while below 100 Kda all the isolates recognized the different molecular weight bands. Out of 20 reacting bands, 5 main bands were detected, 29, 66, 84, 95 and 115 kDa gave positive percent of 60, 60, 60, 90 and 80% among the 10 isolates respectively
Search on Google
Index:
IMEMR (Eastern Mediterranean)
Main subject:
Rabbits
/
Immunoblotting
/
Electrophoresis, Polyacrylamide Gel
/
Animals, Laboratory
/
Antigens
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2005
Similar
MEDLINE
...
LILACS
LIS