IgA class entity determination study in secretion from the mucosal immune system compartments

ABSTRACT

Five separated mucosal viscous colloidal solutions were obtained from human saliva, semen, feces, colostroum and urine. These were biurate positive, 2ME resistant with antibody activity during infection. Solutions were designated as mucosal immunoglobulins [MIG]. MIG separation were performed by using 6% polyethylene glycol solution. Rabbit standard polyclonal anti SIgA was obtained through hyperimmunization of rabbit with standard SIgA. MIG solutions were of SIgA class entity as indicated by immunoelectrophoresis and radial immunoprecipition in gel. The use of PEG 6% for separation and class entity determination of MIG are being reported for the first time in this area