HLA-DR binding prediction and experimental evaluation of T-cell epitopes of mycolyl transferase 85B [Ag85B], a major secreted antigen of mycobacterium tuberculosis
Medical Principles and Practice. 2005; 14 (3): 140-6
in English
| IMEMR
| ID: emr-73518
ABSTRACT
To identify T-cell epitopes of Ag85B by analysis of its sequence for prediction to bind HLA-DR alleles and evaluate the predicted peptides for recognition by T cells in antigen-induced proliferation assays. Materials/Subjects and The complete sequence of Ag85B was analyzed for HLA-DR binding prediction to 51 HLA-DR alleles by using a virtual matrix-based prediction program [ProPred]. Synthetic peptides covering the sequence of mature Ag85B were also analyzed for binding to HLA-DR alleles, and evaluated for recognition in antigen-induced proliferation assays with Ag85B-specific T-cell lines established from the peripheral blood mononuclear cells of 10 HLA-DR-heterogeneous tuberculosis patients. The ProPred analysis of the full-length Ag85B [325 aa], signal peptide [40 aa] and the mature protein [285 aa] predicted their binding to 100, 76 and 98% of the 51 HLA-DR alleles, respectively. The analysis of 31 synthetic peptides for binding to HLA-DR alleles showed that 4 of them could bind >50% HLA-DR alleles, and were considered promiscuous. Testing of Ag85B-specific T-cell lines with synthetic peptides showed that all of the T-cell lines responded to one or more peptides of Ag85B, and 9 of the 10 cell lines responded to one or more of the four peptides considered promiscuous for binding to HLA-DR alleles. The ProPred program was useful in predicting the HLA-DR alleles binding regions of Ag85B and identifying the promiscuous peptides recognized by T cells
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Bacterial Proteins
/
T-Lymphocytes
/
HLA-DR Antigens
/
Epitopes, T-Lymphocyte
/
Antigens, Bacterial
Limits:
Humans
Language:
English
Journal:
Med. Princ. Pract.
Year:
2005
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