TT Virus outbreak among workers in wastewater treatment plants
New Egyptian Journal of Medicine [The]. 2005; 33 (1): 30-35
in English
| IMEMR
| ID: emr-73874
ABSTRACT
The role of TT virus [TTV] as a human pathogen and the mode of transmission are unclear. To determine the prevalence of TTV infection and possible fecal-oral route of transmission, 24 wastewater samples from wastewater treatment plants was collected. Fecal samples were obtained from 60 workers in twaste water treatment plants and 15 healthy persons; all fecal samples were from persons non-transfused blood or blood products. TTV in tested samples were detected by nested PCR, using the NG primer sets. DNA sequences were analyzed from PCR products in both directions. TTV-DNA in water samples was 12.5% [3/24]. While, the overall prevalence of TTV in fecal excretion were 11.66% [7/60], and 6.6% [1/15] in workers and control group; respectively. A total of 100 bp PCR fragments were sequenced and compared to sequences derived from the corresponding TTV genome region deposited in GenBank. Present sequencing was most closely related to TTV-like mini virus, complete genome [Accession NC 002195], at nucleotide number 955 to 1021, suggesting related environmental sources of TTV infection. The phylogenetic analysis suggested that present strain might be sub-strain or mutation from the parent gene of TTV-like mini virus. The present of TTV in wastewater workers may be due to the interaction with contaminated environment and increased susceptibility to infectious agents
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Water Pollution
/
Polymerase Chain Reaction
/
Disease Outbreaks
/
Workplace
/
Waste Management
/
Torque teno virus
/
Feces
/
Industrial Waste
Type of study:
Controlled clinical trial
Limits:
Humans
/
Male
Language:
English
Journal:
New Egypt. J. Med.
Year:
2005
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