Chiral separation of triazole compounds that have a potential for anti-cancer activity using high performance liquid chromatography H.P.L.C. on a solid chiral phase

ABSTRACT

The solid phase 3, 5 di-methyl phenyl carbarnate amylose adsorbed on silica gel was used for the enantiomeric [chiral] separation of the derivatives of azole compounds that were prepared in the lab and have a potential for anti-cancer activity and particularly breast cancer. The important role of the solid phases in separating these compounds was studied where it was relied on the phase that contained principally cellulose tri-3, 5 di-methyl phenyl carbamate amylose adsorbed on silica gel which is present in the chirally selective and commercially called column CHIRALCEL-ODH We also researched the importance of the mobile phase in separating the chiral enantiomers of this group of compounds by depending on the polarity of the mobile phase Hexane-Ethanol-Methanol-di-Methylamine [7515100.1] where we obtained the best resolution. We studied the role of the functional groups in the derivatives of the compounds studied in the chiral separation that contained an azole ring connected to an indol ring, in addition to the difference in the position of the indol ring to the azol ring for the prepared compounds I-IV. The results showed clear difference in time between these families where it varied from 2.65 minute to 6.05 minute