Mechanism of CD11b down-regulation from phorbol myristate acetate stimulated polymorphonuclear neutrophils
Saudi Medical Journal. 2005; 26 (10): 1596-1601
in English
| IMEMR
| ID: emr-74688
ABSTRACT
To investigate the mechanism of CD11b down-regulation in phorbol myristate acetate [PMA] stimulated polymorphonuclear leukocytes [PMN]. Purified PMN were stimulated with PMA in the presence, or absence, of various enzyme inhibitors. Following stimulation, PMN CD11b expression was examined by flow cytometry and Western-blotting. The entire work was carried out at Liverpool University between the period of 1998 and 2001. Stimulation of PMN with PMA induced the down-regulation of CD11b by a mechanism involving a combination of an oxidant and a serine protease; most likely the primary granule derived elastase. The present study shows for the first time the cooperation of both oxidants and enzymes in the down-regulation of PMN adhesion receptors
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Tetradecanoylphorbol Acetate
/
Down-Regulation
/
Cells, Cultured
/
Risk Factors
/
Immunity, Innate
/
Neutrophils
Limits:
Humans
Language:
English
Journal:
Saudi Med. J.
Year:
2005
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