Studies on bovine field skin lesions in Fayoum governorate

ABSTRACT

In this study an outbreak in Fayoum Governorate during summer 1998 showed skin lesions, fever [40-41°C] which resembled clinical manifestation of lumpy skin disease [LSD]. The skin lesion were in the form of firm rounded skin nodules which were covered with erected hair. 15 cases among cattle herd have been recorded, samples from all suspected diseased animals were taken. The samples were inoculated on chorioallantoic membrane [CAM] of emberyonated chicken egg [ECE] aged 9-11 days, for several passages and in MDBK tissue culture for isolation. Confirmatory tests were carried out by using standard specific antisera against LSD virus using agar gel immunodiffusion test AGID, immunoperoxidase and immunofluorescent. The positive lesions of CAM [pock lesion] were collected on 10% neutral formalin buffer for istopathological examination