In vitro effect of folic acid and cobalamin [vitamin B12] on adhesion and growth of Giardia lamblia
Iranian Journal of Parasitology. 2006; 1 (1): 47-52
in English
| IMEMR
| ID: emr-77001
ABSTRACT
Giardia lamblia is one of the most common intestinal protozoan parasites infecting human in the world. The goal of this study was searching for in-vitro effect of folic acid and cobalamin on adhesion and growth of G. lamblia as two important mechanisms in the pathogenesis in TYI-S-33 medium. G. lamblia trophozoites were obtained by in- vitro excystation procedure. Three groups of Giardia trophozoites were analyzed control group, G.lamblia was cultured in TYI-S-33 without any vitamin, 2nd group with 0.1 micro g/ml vitamin B12 or folic acid, and 3rd group with 0.5 micro g/ml of vitamin B12 or folic acid. All culture media tubes incubated at 37 °C. After 2 h of incubation, the adherence into borosilicate culture tubes, and after 24 h the growth of trophozoites were measured .The results showed that in vitamin B12 groups, the growth was increased significantly [P? 0.05] but the adherence decreased significantly [P = 0.05]. Folic acid inhibited the growth rate significantly [P = 0.05], but it increased adherence in axenic culture significantly [P = 0.05]. The results showed that vitamin B12 and folic acid altogether might reduce pathogenesis of G. lamblia by reducing adherence and growth, respectively
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Vitamin B 12
/
Tissue Adhesions
/
Giardia lamblia
/
Folic Acid
Limits:
Humans
Language:
English
Journal:
Iran. J. Parasitol.
Year:
2006
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