Molecular characterization of subunit G of the vacuolar ATPase in pathogen dermatophyte trichophyton rubrum
Iranian Journal of Public Health. 2006; 35 (2): 33-37
in English
| IMEMR
| ID: emr-77152
ABSTRACT
Trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. Several properties of this fungus have been investigated so far. However, a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the subunit G of its vacuolar ATPase [V-ATPase]. Pairs of 21 nt primers were designed from highly conserved regions of the V-ATPase subunit G genes in other fungi. Mentioned primers were utilized in PCR using isolated genomic DNA template as well as cytoplasmic RNA of T.rubrum and the PCR and RT-PCR fragments were then sequenced. About 469 nucleotides were sequenced which encoded a polypeptide with 119 amino acids. Nucleotide sequence comparison in gene data banks [NCBI, NIH] for both the DNA and its deduced amino acid sequence revealed significant homology with V-ATPase subunit G genes and proteins of other eukaryotic cells. The amino acid sequence of the encoded protein was about 84% identical to the sequence of V-ATPase subunit G from other fungi. In summary, we have cloned the first V-ATPase subunit G of dermatophytes and characterized it as a member of this gene family in other eukaryotic cells
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
DNA, Fungal
/
RNA, Fungal
/
Polymerase Chain Reaction
/
Reverse Transcriptase Polymerase Chain Reaction
/
Vacuolar Proton-Translocating ATPases
Language:
English
Journal:
Iran. J. Public Health
Year:
2006
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